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Experimental procedures included surgery models such as subdiaphragmatic vagal deafferentation (SDA), specific ablation of the area postrema (AP), and paraventricular nucleus (PVN) lesions, all of which were conducted on Sprague-Dawley (SPDR) rats

The authors systematically evaluated conflicting findings regarding DSIP's sleep-promoting effects, noting that while initial studies showed dramatic increases in slow-wave sleep, subsequent research produced more variable results depending on species, administration route, and baseline sleep states

Tort, F., et al., Lipoic acid biosynthesis defects

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